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. 2020 Apr 16;10:6494. doi: 10.1038/s41598-020-62920-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Nanoparticle Tracking analysis (NTA) of EVs derived from CRL-5908 cells isolated with three different methods: Yellow line indicates EVs isolated with one-step ultracentrifuge method, red line EVs isolated with commercial kit, and blue line EVs isolated with double-step ultracentrifuge method (peak indicated by arrow). EVs have a mean diameter of 133.7 +/− 6.5 nm and mode of 107.5 +/− 1.7 nm. EV-concentration is expressed as numbers of particles per mL, y axis is marked from 1 to 3,5 E10. (b) Western-blot image of CRL-5908 and CCL-185 cells lysates and their respective isolated EVs: EVs lysates showed higher expression of CD9, lower but presence of HSP70, and absence of GM130 in comparison with cell lines lysates.