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. 2020 Apr 16;10:6494. doi: 10.1038/s41598-020-62920-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Scanning electron microscopy analysis. (a) EVs isolated through double ultracentrifugation. (b) Magnification of a single EV after double ultracentrifugation. (c) Histogram showing the diameter of EVs (nm) separated in its frequency with a peak on 100 to 140 nm. (d) Results of single ultracentrifugation at electron microscopy levels, observing protein or salt aggregates but no EVs, in comparison to double-step ultracentrifugation. (e) Electron microscopy analysis of EVs isolated with ExoEasy Kit revealed similar results as double-step ultracentrifugation.