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. 2020 Apr 16;10:6507. doi: 10.1038/s41598-020-63483-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Experimental workflow from collection of samples to pathway enrichment analysis. (B) H&E staining of rat sections [Upper row: lower magnification, Bar = 500 µm, lower row: higher magnification, Bar = 250 µm], Sham and 72 hours, showing the loss of neurons in the CA1 sub-region of the hippocampus after 72 hours of tGCI as compared to the intact Sham operated rats. In the higher magnification images, small pyknotic neurons are observed indicating neuronal apoptosis.