Table 1.
Significantly up- and downregulated proteins in the proteome of AF22 cells resulting from 24 h oAβ treatment.
| Identified Proteins | Protein ID | AF22 T-Test (p-value) | AF22 Fold Change | ADP2 T-Test (p-value) | ADP2 Fold Change |
|---|---|---|---|---|---|
| Amyloid beta A4 protein | A4_HUMAN | 0.0035 | 3.3 | 0.067 | 17 |
| Aspartate aminotransferase, cytoplasmic | AATC_HUMAN | 0.015 | 1.8 | 0.58 | 1.5 |
| Cytoplasmic aconitate hydratase | ACOC_HUMAN | 0.049 | 0.3 | 0.19 | 0 |
| Proteasomal ubiquitin receptor ADRM1 | ADRM1_HUMAN | 0.035 | 3.8 | 0.13 | INF |
| Actin-related protein 2/3 complex subunit 3 | ARPC3_HUMAN | 0.032 | 0.3 | 0.18 | 2.8 |
| Cluster of Isoform Beta of Apoptosis regulator BAX | BAX_HUMAN | 0.015 | 0.4 | 0.65 | 1.2 |
| Bleomycin hydrolase | BLMH_HUMAN | 0.04 | 0.6 | ND | ND |
| BRI3-binding protein | BRI3B_HUMAN | 0.011 | 2.6 | ND | ND |
| 60 kDa heat shock protein, mitochondrial | CH60_HUMAN | 0.039 | 1.3 | 0.66 | 1.1 |
| Cluster of Isoform 2 of Clathrin heavy chain 1 | CLH1_HUMAN | 0.041 | 0.7 | 0.52 | 1.2 |
| Cluster of Cofilin-1 | COF1_HUMAN | 0.024 | 0.7 | 0.97 | 1 |
| Coatomer subunit delta | COPD_HUMAN | 0.026 | 0.2 | 0.88 | 1.2 |
| Casein kinase II subunit alpha | CSK21_HUMAN | 0.029 | 0.5 | 0.54 | 1.5 |
| Dihydropyrimidinase-related protein 5 | DPYL5_HUMAN | 0.047 | 1.4 | 0.3 | 1.4 |
| Cluster of Spliceosome RNA helicase DDX39B | DX39B_HUMAN | 0.022 | 0.5 | 0.58 | 0.7 |
| Cluster of Isoform 2 of Elongation factor 1-delta | EF1D_HUMAN | 0.038 | 1.6 | 1 | 1 |
| Cluster of Isoform 2 of Histone deacetylase 2 | HDAC2_HUMAN | 0.027 | 0.4 | 0.21 | 0.05 |
| Heterogeneous nuclear ribonucleoprotein F | HNRPF_HUMAN | 0.0087 | 0.5 | 0.78 | 0.9 |
| LIM and SH3 domain protein 1 | LASP1_HUMAN | 0.038 | 2.8 | 0.17 | 3.6 |
| Leucine-rich PPR motif-containing protein, mitochondrial | LPPRC_HUMAN | 0.017 | 0.07 | 0.43 | 0.4 |
| Microtubule-associated protein RP/EB family member 1 | MARE1_HUMAN | 0.015 | 2 | 0.9 | 1.1 |
| Isoform 2 of RNA-binding protein Musashi homolog 2 | MSI2H_HUMAN | 0.037 | 0.5 | 0.47 | 0.2 |
| Metallothionein-3 | MT3_HUMAN | 0.033 | 9.8 | ND | ND |
| Isoform 2 of NADH dehydrogenase 1 alpha subcomplex subunit 11 | NDUAB_HUMAN | 0.015 | 0.3 | 0.34 | INF |
| Isoform 2 of Nuclear mitotic apparatus protein 1 | NUMA1_HUMAN | 0.03 | 3.8 | ND | ND |
| Isoform 2 of GPI transamidase component PIG-S | PIGS_HUMAN | 0.042 | 0.5 | 0.91 | 0.9 |
| Isoform 2 of Proteasome subunit alpha type-3 | PSA3_HUMAN | 0.038 | 1.8 | 0.13 | 4 |
| Cluster of 26 S proteasome non-ATPase regulatory subunit 4 | PSMD4_HUMAN | 0.046 | 2.6 | 0.21 | 3.3 |
| Multifunctional protein ADE2 | PUR6_HUMAN | 0.04 | 0.5 | 0.99 | 1 |
| Cluster of 60 S ribosomal protein L26 | RL26_HUMAN | 0.018 | 0.3 | 0.22 | 2.4 |
| Small glutamine-rich tetratricopeptide repeat-containing protein alpha | SGTA_HUMAN | 0.018 | 1.9 | 0.31 | 1.5 |
| Synaptogyrin-3 | SNG3_HUMAN | 0.036 | 2.1 | 0.32 | 2.6 |
| Signal recognition particle subunit SRP72 | SRP72_HUMAN | 0.045 | 0 | 0.34 | 0 |
| Translocon-associated protein subunit delta | SSRD_HUMAN | 0.022 | 0.5 | 0.61 | 0.8 |
| Phenylalanine–tRNA ligase alpha subunit | SYFA_HUMAN | 0.037 | 0.5 | 0.8 | 0.8 |
| TAR DNA-binding protein 43 | TADBP_HUMAN | 0.038 | 0.4 | 0.64 | 0.8 |
| Transformer-2 protein homolog beta | TRA2B_HUMAN | 0.047 | 0.4 | 0.27 | 0.5 |
| Cluster of Isoform 3 of Thioredoxin reductase 1, cytoplasmic | TRXR1_HUMAN | 0.041 | 0.4 | 0.11 | 3.8 |
| Isoform 3 of 116 kDa U5 small nuclear ribonucleoprotein component | U5S1_HUMAN | 0.03 | 0.2 | 0.52 | 0.5 |
| Isoform 3 of Vesicle-associated membrane protein 1 | VAMP1_HUMAN | 0.039 | 87 | 0.99 | 1 |
| Cluster of Vesicle-associated membrane protein 2 | VAMP2_HUMAN | 0.036 | 1.5 | 0.29 | 1.5 |
The provided table depicts proteins with significant changes in expression (p < 0.05, T-test with Benjamini-Hochberg correction) in cells challenged with oAβ relative to untreated cells (i.e. AF22 + oAβ relative to AF22 control). AF22: n = 16 (control), n = 14 (oAβ treated). Data represent analysis of whole proteome preparations. ADP2 columns provided for comparison. ADP2: n = 7 (control), n = 7 (oAβ treated). ND = Not detected.