Table 4.
Phosphorylation at Serine 208 is differentially expressed after 24 h oAβ challenge and may be a useful residue in the detection of early oAβ-induced effects.
| Tau P10636-8 AA 441 | Control samples with phosphorylation detected at residue: (n = 11) | Percentage of Control with detected residue | oAβ challenged samples with phosphorylation detected at residue: (n = 8) | Percentage of oAβ challenged with detected residue |
|---|---|---|---|---|
| 181 | 1 | 9.09090909 | 1 | 12.5 |
| 197 | 2 | 18.1818182 | 2 | 25 |
| 198 | 2 | 18.1818182 | 2 | 25 |
| 199 | 8 | 72.7272727 | 6 | 75 |
| 202 | 11 | 100 | 8 | 100 |
| 205 | 3 | 27.2727273 | 3 | 37.5 |
| 208 | 3 | 27.2727273 | 6 | 75 |
| 212 | 1 | 9.09090909 | 0 | 0 |
| 217 | 1 | 9.09090909 | 0 | 0 |
| 231 | 2 | 18.1818182 | 0 | 0 |
| 235 | 9 | 81.8181818 | 7 | 87.5 |
| 238 | 2 | 18.1818182 | 0 | 0 |
| 262 | 3 | 27.2727273 | 1 | 12.5 |
| 394 | 1 | 9.09090909 | 0 | 0 |
| 396 | 5 | 45.4545455 | 5 | 62.5 |
| 400 | 9 | 81.8181818 | 8 | 100 |
| 403 | 2 | 18.1818182 | 0 | 0 |
| 404 | 11 | 100 | 8 | 100 |
Canonical phosphorylation sites such as 181, 202, 231, 262 and 404 do not show any appreciable changes at this early stage. The table depicts all detected phosphorylated tau residues for both AF22 cells challenged with oAβ and untreated AF22 cells. Phosphorylations detailed in this table were identified after TiO2 enrichment. Amino acid number corresponds to full-length 2N4R tau (441 residues; UniProt accession P10636-8), n = 11 (control) and n = 8 (oAβ).