Fig. 7.

CCL-5 and PD-L1 antibody combined blockade increases overall survival of the orthotopic model of pancreatic cancer. a C57BL/6 mice were orthotopically injected with Pan02-pLV-control-Luc (lentivirus vectors with luciferase gene stably expressed in Pan02 cells) or Pan02-pLV-FOXP3-Luc (lentivirus vectors with FOXP3 and luciferase gene stably expressed in Pan02 cells). Mice were randomized and treated with anti-CCL5, anti-PD-L1, anti-CCL5 + anti-PD-L1, anti-CCL5 + anti-PD-L1 + anti-CD8 or isotype control Abs until the study endpoint. b (left) Tumor growth was assessed by bioluminescent imaging. (right) Quantification of the bioluminescent imaging signals on day 5 and day 9. The results are expressed as the fold change relative to those on day 5. Histogram (columns: mean; bars: standard deviation, n = 5), p values were calculated by paired T-test. *p < 0.05, **p < 0.01. c Kaplan–Meier survival curves with log-rank test for significance between different groups (*p < 0.05, **p < 0.01)