Fig. 2.

Mitochondria staining in C2C12 cells after 7 days culture with various copolymers in myogenic differentiation medium. (A) Fluorescent staining of mitochondria by Mito-tracker (scale bar 200 μm); (B) Quantitative analysis of the mitochondrial amount per cell on Mito-tracker green fluorescent staining by image J. TCP was the blank control, all experiments were performed in triplicate (n = 4 per group, *p < 0.05, **p < 0.01). POCG-PEI600 copolymers significantly enhanced the mitochondrial number in C2C12 myoblasts. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)