Fig. 4.

Molecular mechanism analysis of myogenic differentiation of copolymers (40 μg/mL). (A) Immunofluorescence staining of MHC protein (red) in C2C12 cells on day 7 after incubation with copolymers in inhibitor-added myogenic differentiation medium (scale bar 200 μm, cell nuclei: blue); (B) Western blot analysis of MAPK pathway related proteins in C2C12 cells on day 7 after incubation with copolymers in inhibitor-added myogenic differentiation medium; (C–F) Quantitative gray level based on the Western blot bands for P-p38 (C), p38 (D), MHC (E) and MYOD (F) proteins expression; (G) Myod and Mhc gene expression in C2C12 cells on day 7 after incubation with POCG-PEI600 copolymers in inhibitor-added myogenic differentiation medium. All experiments were performed in triplicate (n = 4 per group, *p < 0.05, **p < 0.01). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)