Fig. 5.

In vivo skeletal muscle tissue regeneration evaluation of POCG-PEI600 copolymer after implanted on the full-thickness muscle defect mice model for 7 days. (A) Optical image of skeletal muscle defect on day 7, normal skeletal muscle was used as a control; (B) H.E. staining pictures of treated skeletal muscle tissue (scale bars = 50 μm, black arrow: centronucleated myofibers); (C) Analysis of formed myofibers diameter in the defect area; (D) Statistical data of capillary density in the regenerative site; (E) Numbers of centronucleated myofibers in repaired tissue; (F) Myod and Mhc gene expression in repaired muscle tissues. The empty and commercial F127 hydrogel treated groups were used as controls, *p < 0.05. **p < 0.01.