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. 2020 Mar 31;17:107–117. doi: 10.1016/j.omto.2020.03.016

Figure 4.

Figure 4

OBP-301 and OBP-702 Inhibit the Migration and Invasion of Highly Invasive PDAC Cells Stimulated with Neurosecretory Factors

(A) Migration assay using highly invasive PDAC cells stimulated with norepinephrine (NE), nerve growth factor (NGF), or glial cell line-derived neurotrophic factor (GDNF) at the indicated doses. ∗p < 0.05 (versus control). (B) Expression of phospho-ERK1/2 (p-ERK1/2) and ERK1/2 proteins in highly invasive PDAC cells stimulated with NE (100 μM), NGF (100 ng/mL), or GDNF (100 ng/mL) for 24 h. β-Actin was assayed as a loading control. (C) Migration and invasion assay using highly invasive PDAC cells stimulated with NE (100 μM), NGF (100 ng/mL), or GDNF (100 ng/mL) for 24 h in the presence of OBP-301 (100 MOI) or OBP-702 (100 MOI). The number of migrating and invading cells after treatment was calculated relative to that of non-treated cells, which was set at 1.0. Data are expressed as mean ± SD (n = 5). ∗p < 0.05 (versus NE, NGF, or GDNF).