Figure 5.
OBP-301 and OBP-702 Inhibit Tumor Growth in a Subcutaneous BxPC-3 Tumor Model
(A) BxPC-3 cells (5 × 106 cells/site) were inoculated into the right flanks of athymic nude mice. OBP-301 (108 PFUs), OBP-702 (108 PFUs), or PBS (mock) was injected into the tumors on days 0, 2, and 4. Data are expressed as mean tumor volume ± SD (mock group: n = 8; OBP-301 and OBP-702 groups: n = 9). (B) Macroscopic appearance of representative mice on day 37 after first treatment. (C) Macroscopic appearance of all isolated tumors on day 37 after first treatment. (D) Histologic analysis of BxPC-3 tumors. Paraffin-embedded sections of BxPC-3 tumors were stained with hematoxylin and eosin (H&E) or anti-Ki-67 antibody. Left two images and right two images represent H&E and Ki-67 staining, respectively, with low (×100) and high (×400) original magnification. High-magnification images are the area outlined by a black square in the low-magnification images. (E) Percentage of Ki-67-positive cells in each group. The percentage of Ki-67-positive cells was calculated from three random fields of tumor section in each group under microscopy. ∗p < 0.05.