Figure 4.

Effect of circFUT10 on the cell proliferation

(A) Visualization of the efficiency of circFUT10 overexpression vector pCD2.1-circFUT10 by qPCR. (B and C) Cell proliferation analysis using a Cell Counting Kit-8 (CKK-8) assay (B) and EdU incorporation assays (C). Scale bars represent 100 mm. (D and E) Bovine primary fat cells were transfected with pCD2.1-circFUT10, cell phases were analyzed by flow cytometry (E) and made statistics (D). (F and G) The expression of proliferating cell nuclear antigen (PCNA) and cyclin-dependent-kinase 2 (CDK2) were detected by qPCR (F) and western blot (G); quantitative analysis of western blot was performed using ImageJ. Values are mean ± SEM for three biological replicates. ∗p < 0.05, ∗∗p < 0.01.