Figure 6.

circFUT10 Acts as a ceRNA for let-7c

(A) let-7b, let-7c, let-7e, and let-7i were co-transfected with pCK-circFUT10 into HEK293T cells (eight replicates in each group, each replicate adding 0.1 μg of miRNA plasmid and 0.1 μg of pCK-circFUT10 plasmid). Renilla luciferase activity was normalized to firefly luciferase activity. (B) let-7c sensor construct and co-transfected with let-7c mimic or pcDNA3.1-circFUT10 into HEK293T cells. (C) Schematic diagram of the let-7c sensor structure. (D) Association of circFUT10 and let-7c with Ago2. circFUT10 and let-7c were transfected into adipocytes for 24 h and lysed; cellular lysates were used for the RIP assay with Ago2 antibody. circFUT10 and let-7c levels were detected using qPCR. ∗p < 0.05, ∗∗p < 0.01.