Figure 7.

circFUT10 Binding of let-7c Promotes Adipocyte Proliferation and Inhibits Its Differentiation

(A and B) Fat cells were co-transfected with the let-7c mimic and circFUT10; the expression levels of proliferating cell nuclear antigen (PCNA) and cyclin-dependent-kinase 2 (CDK2) were detected by qPCR (A) and western blot (B). (C and D) The expression levels of PPARγ and C/EBPβ in bovine adipocytes were measured by qPCR (C) and western blot (D). (E and F) Oil red O staining was detected after fat cells were co-transfected with the let-7c mimic and circFUT10 (E). Quantitative analysis of western blot and oil red O were performed using ImageJ (F). Control, 1 μg of pCD2.1 plasmid + 2.5 μL of mimic NC; circFUT10, 1 μg of circFUT10 plasmid + 2.5 μL of mimic NC; let-7c, 1 μg of pCD2.1 plasmid + 2.5 μL of let-7c mimic; circFUT10+let-7c, 1 μg of circFUT10 plasmid + 2.5 μL of let-7c mimic. ∗p < 0.05, ∗∗p < 0.01.