Figure 8.

PPARGC1B Is a Target of let-7c

(A) Predicted binding sites of let-7c in the 3′ UTR of PPARGC1B. The sequences indicated in red indicate certain mutated bases. (B) HEK293T cells were co-transfected with the wild-type or mutant PPARGC1B dual-luciferase vector and let-7c mimic, and the relative luciferase activity was analyzed 24 h after transfection. (C) The let-7c markedly inhibited PPARGC1B expression, and this effect was abrogated by circFUT10 overexpression. (D) The circFUT10 markedly promoted PPARGC1B expression, and this effect was abrogated by let-7c overexpression. ∗p < 0.05, ∗∗p < 0.01.