FIG 1.

Gating strategy for assessing colonic ILCs and NKs of uninfected and SIV-infected rhesus macaques. (A and B) The gating strategy for defining ILCs and NKs from uninfected (A) and infected (B) colons involved selecting CD45-expressing, single cells that were viable. CD45⁺ viable single cells, which were within the lineage (Lin; CD3, CD11c, CD20, CD34, CD123, CD303, FCεR1) gate, were excluded from our analysis. Cells outside the Lin gate were further evaluated for cells expressing CD127. Cells lacking CD127 but possessing CD56 were considered CD56⁺ NKs. The CD127-expressing cells were evaluated for CD117 expression. Cells within the CD127 gate, which were CD117⁺, were ILC3s and ILC2s, whereas the cells lacking CD117 were ILC1s. FSC, forward scatter; SSC, side scatter. Fluorescent minus one (FMO) controls were used to set gates for determining the frequency of ILCs and NKs from uninfected (C) and SIV-infected (D) colons.