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. 2020 Apr 16;94(9):e00119-20. doi: 10.1128/JVI.00119-20

TABLE 3.

Details of seven novel ASFV candidate genesa

Putative gene Strandb Transcription start site (nt)c Transcription end site (nt)d Putative protein length (aa) Similarity according to NCBI BLAST
Gene-end no. of Ts
Homologous sequence (accession no.) E value
pNG1 + 13053 13435 25 13 residues had 92% identity to ASFV-G-ACD-00350 (AZP54308.1) 0.11 6
pNG2 30091 29827 50 50 residues had 100% identity with ASFV26544 00600 (AKM05534.1) 8
pNG3 + 12664 12896 44 38 residues had 59% identity to ASFV-G-ACD-00290 (AZP54130.1) 0.13 6
pNG4 + 10583 10835 44 42 residues had 65% identity with ASFV-G-ACD-00290 (AZP54130.1) 1e−09 6
pNG5 + 29817 30080 31 No significant similarity None
pNG6 + 167005 167336 56 56 residues aligned with 40% identity to pKP93L (AIY22188.1) 6e−07 5
pNG7 + 10484 10616 32 32 residues aligned with a 31-aa hypothetical protein with from ASFV Belgium 2018/1 with 87% identity (VFV47940.1)e 8e−10 3
a

NCBI ORFfinder and BLAST were used to predict the putative encoded ORFs which were subsequently analyzed for putative homologous sequences (88, 89).

b

Plus (+) and minus (−) strands are indicated.

c

Defined as a pTSS from CAGE-seq analysis.

d

Defined from 3′ RNA-seq analysis. Underlined transcription ends were defined from only RNA-seq. pNG5 is in the antisense orientation relative to pNG2, and the RNA-3′ end of pNG6 is dispersed according to RNA-seq and may overlap DP42R. pNG7 overlaps pNG4 on the same strand.

e

From BioProject PRJEB31287.