TABLE 3.

Details of seven novel ASFV candidate genes^a

Putative gene	Strandb	Transcription start site (nt)c	Transcription end site (nt)d	Putative protein length (aa)	Similarity according to NCBI BLAST		Gene-end no. of Ts
					Homologous sequence (accession no.)	E value
pNG1	+	13053	13435	25	13 residues had 92% identity to ASFV-G-ACD-00350 (AZP54308.1)	0.11	6
pNG2	−	30091	29827	50	50 residues had 100% identity with ASFV26544 00600 (AKM05534.1)		8
pNG3	+	12664	12896	44	38 residues had 59% identity to ASFV-G-ACD-00290 (AZP54130.1)	0.13	6
pNG4	+	10583	10835	44	42 residues had 65% identity with ASFV-G-ACD-00290 (AZP54130.1)	1e−09	6
pNG5	+	29817	30080	31	No significant similarity		None
pNG6	+	167005	167336	56	56 residues aligned with 40% identity to pKP93L (AIY22188.1)	6e−07	5
pNG7	+	10484	10616	32	32 residues aligned with a 31-aa hypothetical protein with from ASFV Belgium 2018/1 with 87% identity (VFV47940.1)e	8e−10	3

^aNCBI ORFfinder and BLAST were used to predict the putative encoded ORFs which were subsequently analyzed for putative homologous sequences (88, 89).

^bPlus (+) and minus (−) strands are indicated.

^cDefined as a pTSS from CAGE-seq analysis.

^dDefined from 3′ RNA-seq analysis. Underlined transcription ends were defined from only RNA-seq. pNG5 is in the antisense orientation relative to pNG2, and the RNA-3′ end of pNG6 is dispersed according to RNA-seq and may overlap DP42R. pNG7 overlaps pNG4 on the same strand.

^eFrom BioProject PRJEB31287.