TABLE 3.
Details of seven novel ASFV candidate genesa
Putative gene | Strandb | Transcription start site (nt)c | Transcription end site (nt)d | Putative protein length (aa) | Similarity according to NCBI BLAST |
Gene-end no. of Ts | |
---|---|---|---|---|---|---|---|
Homologous sequence (accession no.) | E value | ||||||
pNG1 | + | 13053 | 13435 | 25 | 13 residues had 92% identity to ASFV-G-ACD-00350 (AZP54308.1) | 0.11 | 6 |
pNG2 | − | 30091 | 29827 | 50 | 50 residues had 100% identity with ASFV26544 00600 (AKM05534.1) | 8 | |
pNG3 | + | 12664 | 12896 | 44 | 38 residues had 59% identity to ASFV-G-ACD-00290 (AZP54130.1) | 0.13 | 6 |
pNG4 | + | 10583 | 10835 | 44 | 42 residues had 65% identity with ASFV-G-ACD-00290 (AZP54130.1) | 1e−09 | 6 |
pNG5 | + | 29817 | 30080 | 31 | No significant similarity | None | |
pNG6 | + | 167005 | 167336 | 56 | 56 residues aligned with 40% identity to pKP93L (AIY22188.1) | 6e−07 | 5 |
pNG7 | + | 10484 | 10616 | 32 | 32 residues aligned with a 31-aa hypothetical protein with from ASFV Belgium 2018/1 with 87% identity (VFV47940.1)e | 8e−10 | 3 |
NCBI ORFfinder and BLAST were used to predict the putative encoded ORFs which were subsequently analyzed for putative homologous sequences (88, 89).
Plus (+) and minus (−) strands are indicated.
Defined as a pTSS from CAGE-seq analysis.
Defined from 3′ RNA-seq analysis. Underlined transcription ends were defined from only RNA-seq. pNG5 is in the antisense orientation relative to pNG2, and the RNA-3′ end of pNG6 is dispersed according to RNA-seq and may overlap DP42R. pNG7 overlaps pNG4 on the same strand.
From BioProject PRJEB31287.