FIG 1.

Murine RV VP3 confers resistance to IFN antiviral activity in MEFs. WT and Ifnar1^−/− Ifngr1^−/− MEFs were pretreated with or without 1,000 U/ml murine IFN-β for 24 h and then infected with the indicated RV strains (MOI = 1). At 24 h postinfection (hpi), virus-infected cells were harvested, and virus yields were determined by a focus-forming unit (FFU) assay. Fold differences in virus titers were calculated as follows: yield of WT or Ifnar1^−/− Ifngr1^−/− MEFs with IFN treatment (FFU per milliliter)/yield of MEFs without IFN treatment. The fold differences in virus titers presented are the means of quadruple data points. R represents viral genes from the simian RV RRV strain, and E represents viral genes from the murine RV EW strain.