FIG 2.

RNase L signaling inhibits simian RV replication in vitro and in vivo. (A) WT and Rnasel^−/− MEFs were pretreated with or without 1,000 U/ml murine IFN-β for 24 h and then infected with simian RRV or murine ETD RV at an MOI of 1 for 24 h. Virus yields were determined by a focus-forming unit assay. Fold differences in virus titers were calculated as follows: yield of WT or Rnasel^−/− MEFs with IFN treatment (FFU per milliliter)/yield of MEFs without IFN treatment. The fold differences in virus titers presented are the means of quadruple data points. n.s., not significant. (B) Five-day-old WT and Rnasel^−/− pups were orally inoculated with simian RRV (10⁷ PFU) or murine EW (10⁴ DD₅₀). Fecal specimens were collected on the indicated days postinfection and subjected to a real-time PCR-based assay measuring RV gene NSP5 levels with standard curves to determine infectious virus particles per gram of stool sample. The numbers of mice in each group are indicated in parentheses.