FIG 5.

VP3 PDE mutations minimally affect RV replication in MA104 cells. (A) Schematic diagram of the recombinant simian RV SA11 strain rescued using reverse genetics. MTase, methyltransferase; RTPase, RNA 5′-triphosphatase. (B) MA104 cells were infected by recombinant WT or VP3 mutant RVs at an MOI of 1. At the indicated time points, total RNA was harvested, and the RV gene NSP5 level was measured by reverse transcription-quantitative PCR (RT-qPCR) and normalized to the value for GAPDH.