FIG 6.

RV VP3 PDE activity contributes to viral replication in HT-29 cells. (A) WT HT-29 cells were transfected with 4 μg/ml of poly(I·C) for 1 h and subsequently infected with recombinant WT or VP3 mutant RVs at an MOI of 10 for 9 h. Total RNA was extracted and resolved by an RNA chip assay. (B) Quantification of intact 18S and 28S rRNA bands and RNase L cleavage products in panel A. The arithmetic means ± standard deviations from three independent experiments are shown. (C) WT and RNASEL^−/− HT-29 cells were infected with recombinant WT or VP3 mutant RVs at an MOI of 1 for 24 h. Virus particles in the supernatant were titrated by an FFU assay.