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Dynamics of total and intact HIV DNA levels as determined by IPDA in rCD4 and aCD4 T cells after in vitro infection. Cells were either infected with NL4-3 or activated for 3 days with CD3/CD28 beads before infection. Cells were cultured with SQV and collected at 2, 4, 8, 24, 48, 96, and 192 h to measure total HIV DNA by qPCR and intact HIV DNA by a modified IPDA. Total and intact HIV DNA followed a similar pattern in terms of both peak infection and decay over time in culture.
