FIG 2.

Correlates of protection in RdRP mice during acute-phase infection. Total RNA isolated from spleen (A, B, and C) and bone marrow (D, E) from infected and uninfected mice described in Fig. 1 was used for reverse transcription-quantitative PCR (qRT-PCR) analysis of differences in mRNA expression. (A) ISGs Ifit1 and Oasl2 in spleen. We observed a similar baseline 5- to 10-fold upregulation of Isg15 (interferon-stimulated gene 15) mRNA in uninfected RdRP mice spleens (data not shown). (B) RdRP mRNA expression. (C) The proinflammatory cytokines TNF-α and IL-6 were similarly assessed. For experiments shown, n = 6 for WT uninfected and WT, n = 5 for RdRP uninfected, and n = 7 for RdRP. (D, E) Similar assays for ISG expression (D) or proinflammatory cytokine expression (E) were performed on bone marrow infected with 1,000-PFU B-tropic FV, harvested on 7 dpi; n = 4 for uninfected WT, n = 5 for uninfected RdRP, and n = 7 for infected WT and RdRP. Data were analyzed using a one-way ANOVA followed by a Tukey test for individual comparison (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001).