FIG 5.

RdRP mice are resistant to low-dose FV during acute-phase time points. (A) Design of mouse infection experiments to determine recovery in RdRP mice. Mixed-gender mice 16 to 18 weeks of age were infected with 1,000 PFU virus and harvested for disease progression analysis at 1 and 8 weeks after infection to assess matched acute and chronic disease. (B) Spleen weight harvested at 1 week postinfection. (C, E) Single-cell suspensions of splenocytes stained for overall FV infection level (C) and specific infection levels in erythroblasts, B cells, T cells, and DCs, determined by flow cytometry (E). (D, F) Single-cell suspensions of bone marrow cells were stained for overall infection (D) and specific infection in erythroblasts, B cells, T cells, and DCs (F), and analyzed by flow cytometry as in panels C and E. In all experiments, n = 4 for uninfected WT, n = 5 for uninfected RdRP, and n = 7 for both WT and RdRP. A one-way ANOVA followed by a Tukey test to determine significance was used to analyze spleen weight in panel B, and for all other analyses, a Student's t test was used to determine significance. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.