TABLE 4.
Summary of vaccine-elicited immune responses at week 20a
| Category | Median response magnitude | Value for the following vaccine: |
||
|---|---|---|---|---|
| gB FL | gB ecto | gB mRNA | ||
| gB binding | gB FL binding (log10 MFI) | 2.85 | 3.37 | 3.53 * |
| gB ecto binding MFI (log10 MFI) | 2.77 | 3.19 | 3.34 * | |
| Cell-associated gB binding (% of cells) | 18.81 | 18.58 | 29.05 * | |
| RAI (gB FL target) | 0.93 | 0.90 | 0.78 | |
| Peptide bindingb | Total binding sum (log10 MFI) | 5.25 | 4.74 | 5.04 |
| AD-3 binding sum (log10 MFI) | 5.12 | 2.27 | 4.77 | |
| No. of peptides bound (>100 MFI) | 44.5 | 28.5 | 85 * | |
| Neutralizing epitope binding and neutralization | Domain 1 binding (log10 MFI) | 2.76 | 3.37 | 3.44 * |
| Domain 1 + 2 binding (log10 MFI) | 2.96 | 3.49 | 3.03 | |
| AD-1 binding (log10 MFI) | NMR | NMR | NMR | |
| AD-2 binding (log10 MFI) | NMR | NMR | NMR | |
| AD169r Fibro Neut + C (log10 ID50) | 1.69 | 1.79 | 2.08 | |
| AD169r Epi Neut + C (log10 ID50) | 1.91 | 1.83 | 2.29 | |
| Fcγ receptor binding and function | FcγRI binding (log10 MFI) | 3.22 | 3.41 | 3.59 |
| FcγRIIa binding (log10 MFI) | 3.14 | 3.24 | 3.29 | |
| FcγRIIb binding (log10 MFI) | 1.71 | 2.27 | 3.07 | |
| FcγRIIIa binding (log10 MFI) | 3.59 | 3.69 | 4.04 * | |
| TB40/E virion phagocytosis (% of cells) | 10.06* | 7.04 | 7.06 | |
| NK cell degranulation (% of cells) | NMR | NMR | NMR | |
| T cells | Splenic gB-specific T cells (% of live T cells) | 0.22 | NMR | 0.38 |
a
Data in boldface indicate the maximal response. Fibro Neut, neutralization in fibroblasts; Epi Neut, neutralization in epithelial cells; +C, in the presence of complement; NMR, no measurable response above the baseline levels; *, P < 0.05, Kruskal-Wallis test with the post hoc Mann-Whitney U test (compared to the results for gB FL).
b
Peptide binding using week 10 sera.