TABLE 1.
List of primers
| Primer function and target DNAa | Forward (5′ → 3′) | Reverse (5′ → 3′) |
|---|---|---|
| FISH | ||
| C | ATGGGTCTGAAATATTTCTC | TTAATTAATTACAATCTCC |
| M | GCTGCGTATCAAGACGAC | TTCTAGCATCCCAATTCCTTTC |
| N | TGGCAGATTGGTTTTCTAGT | TTCTGAGTGAATGTACAATAAACATTT |
| R | ACATTAAATAATCCTCTCTCTCCTA | CCTATCATCACTAAACATGCC |
| S | AAATGGTGAGCAATTGGAA | GCCTATGATAGTAATCATATCTTGACA |
| U1 | TTGGTCGATTATTTGTTGGTT | AATATCTCATTAGCATTAATTACATTTGAA |
| U2 | TTATGGATGCCGGCTTT | CATCAAGTATTAGAATAACGAACTTGA |
| U4 | AGCAGGTTATCGAATGTAG | ATAGATTCCCACAATCGCT |
| Mutagenesis | ||
| N-His | CACCACCACGCAGATTGGTTTTCTAGTC | ATGATGATGCATTTTTCTGCAACTTCC |
| His-N | CACCACCACTAATTAGTTGTGATGATGTAATTAATAATAATT | ATGATGATGCACTTTGATTCTGAGTGAATG |
| Control His | ||
| ATG-His | ATGCATCATCATCACCACCAC | GTTCCTGTTTCCACCATAGAAACTAC |
| His-TAA | GCATGAAAGACAAGCTCAACG | TTAGTGGTGGTGATGATGATG |
a
Primers were used to amplify the coding sequence of each segment for segment-specific fluorescent labeling during FISH, to generate the pCambia 2300-His-N-SL or pCambia 2300-N-His-SL (mutagenesis), and to control the presence of DNA-His-N or DNA-N-His in infected plants (control His).