FIG 2.

Cytotoxicity and anti-EV71 activity of FNC and its 7 analogs. (A) The dose-dependent cytotoxicity profile of the 8 compounds was detected using a CCK-8 kit in RD cells at 48 h. (B) Anti-EV71 activities of the 8 compounds in the safe-dose range. RD cells were infected with EV71 at an MOI of 0.1 for 4 h, washed twice with DMEM, and supplemented with DMEM containing the indicated concentration of the 8 compounds for another 48 h. The cells were harvested for Western blot (WB) analysis, and tubulin was used as a loading control. (C) Cytotoxicity of FNC in RD cells was detected at 0 to 1 μM at 48 h. (D) The CC₅₀ of FNC in RD cells was calculated using GraphPad Prism7.