FIG 9.

FNC protects neonatal mice from EV71 and CA16 lethal challenges. (A to F) One-day-old ICR mice (n = 8 to 10) were inoculated with 10 μl of EV71 CC063 at 10^5.5 CCID₅₀ ml⁻¹ (A, C, and E) or CA16 CC045 at 10^2.7 CCID₅₀ ml⁻¹ (B, D, and F) and at 1 h postchallenge injected intraperitoneally with FNC at days 1, 3, 6, 9, and 12. Clinical scores and survival rates were monitored for 15 days postchallenge. Various grades of clinical disease were identified as follows: 0, healthy; 1, lethargy and inactivity; 2, wasting; 3, limb-tremor weakness; 4, hind-limb paralysis; and 5, moribund or dead. (E and F) Viral loads of EV71 (E) and CA16 (F) in samples of the brain, lung, spine skeletal muscle, and hind-limb muscle tissues of infected mice were assessed by RT-qPCR at day 8. The results represent the mean virus loads (log₁₀ copies/mg tissue) ± SDs (three mice per group, repeated three times).