TABLE 1.
Primers used for RT-qPCR in this study
| Primer name | Primer direction | Sequence (5′–3′) |
|---|---|---|
| EV71-RT-F | Forward | CTTTGTGCGCCTGTTTTATAC |
| EV71-RT-R | Reverse | GGAAACAGAAGTGCTTGATCA |
| CA16-RT-F1 | Forward | CATGCAGCGCTTGTGCTT |
| CA16-RT-F2 | Forward | CATGCAACGACTGTGCTTTC |
| CA16-RT-R1 | Reverse | CACACAATTCCCCCGTCTTACT |
| CA16-RT-R2 | Reverse | CATAATTCGCCCGTTTTGCT |
| CA6-RT-F | Forward | AATGAGGCGAGTGTGGAAC |
| CA6-RT-R | Reverse | AGGTTGGACACAAAAGTGAACT |
| CVB3-RT-F | Forward | GAATGCGGCTAATCCTAACTGC |
| CVB3-RT-R | Reverse | GCTCTATTAGTCACCGGATGGC |
| EVD68-RT-F | Forward | CAGTCACAGCCACACTAGC |
| EVD68-RT-R | Reverse | CAATCTAAACCCCTGAGAGC |
| Negative-sense-RT-F | Forward | TTAAAACAGCCTGTGGGTTG |
| Positive-sense-RT-R | Reverse | Oligo d(T) |
| RSV-G-RT-F | Forward | ACCTGCTGGGCTATCTGC |
| RSV-G-RT-R | Reverse | TTGGTTGTCTTGAGGGTTG |
| RSV-N-RT-F | Forward | TGCAGGGCAAGTGATGTTAC |
| RSV-N-RT-R | Reverse | TTCCATTTCTGCTTGCACAC |
| RSV-M2-1-RT-F | Forward | TGGCCACCCCATGCACTG |
| RSV-M2-1-RT-R | Reverse | TCCAACTCTGCAGCTCCAC |
| RSV-SH-RT-F | Forward | ACATGATCACAACAATAATCTC |
| RSV-SH-RT-R | Reverse | AAAGGTTTTGTTATGGAATACG |
| H1N1-NP-RT-F | Forward | TGCTTCAAAACAGCCAAGTG |
| H1N1-NP-RT-R | Reverse | GATGCCCTCTGTTGATTGGT |
| GAPDH-RT-F | Forward | TGCACCACCAACTGCTTAGC |
| GAPDH-RT-R | Reverse | GGCATGGACTGTGGTCATGAG |