FIG 2.

The distribution of dsRNA, RIG-I, and NP in pathogenic arenavirus-infected cells. A549 cells were infected with rJUNV (pathogenic Romero strain), rMACV, or rLASV (MOI, 1.0). (A) At 48 hpi, cells were fixed and stained for dsRNA (green), RIG-I (red), JUNV, MACV, or LASV NPs (magenta), and nucleus (blue). Images are representative of 3 separate experiments. Arrows indicate areas showing dsRNA, RIG-I, and NP triple colocalization. White lines are drawn in the JUNV, MACV, and LASV samples. The fluorescence plot profiles of signal intensities for NP (purple), dsRNA (green), and RIG-I (red) around the white lines are shown. (B) Pearson’s colocalization coefficients of dsRNA and RIG-I in JUNV and MACV infections. (C) RT-qPCR analysis of ISG15 mRNA levels. The mRNA levels were normalized to β-actin mRNA levels and are presented as fold changes to mock samples (mean + standard deviation [SD]) from three experiments. (D) Western blotting of LASV, JUNV, and MACV NPs, ISG15, and β-actin.