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. 2020 Apr 16;94(9):e01567-19. doi: 10.1128/JVI.01567-19

FIG 4.

FIG 4

Neu5Gc and O-acetyl Sia modifications vary by tissue in wild-type C57BL/6 mice, and absence of Neu5Gc in CMAH−/− mice leads to an increase in O-acetylation across tissues. Total Sia was measured from tissue samples using HPLC analysis to determine relative Sia quantities. (A) Neu5Gc levels were measured in tissues from WT mice showing variable levels of expression between different tissues. CMAH−/− mice showed undetectable levels of Neu5Gc. Filled cirlces show the individual data points. The error bars indicate standard deviations. (B and C) Percentages of O-acetyl-modified Sia in different tissues from WT (B) and CMAH−/− (C) mice are given as a heat map showing variation across tissues. The white squares indicate that the Sia form was below the limit of detection. Values are given as the percentage of total Sia collected from tissue samples. The sample size for each tissue was three individual mice (n = 3) of each mouse strain, with average values for total sialic acid content given in Tables 1 and 2.