TABLE 1.
Average relative Sia quantities as determined by HPLC analysis for each tissue tested in WT C57BL/6 mice (n = 3)
| Tissue | % Siaa
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Neu5Gc | Neu5Ac | Neu5,9Ac2 | Neu5,7Ac2 | Neu5Gc8Ac | Neu4,5Ac2 | Neu5,8Ac2 | Neu5,7,8/9Ac3 | O-Ac sum | |
| Lung | 50.2 | 47.6 | 1.2 | BD | 1.3 | 0.5 | 0.2 | 0.4 | 3.6 |
| Trachea | 43.0 | 53.5 | 1.3 | BD | 1.5 | 0.4 | 0.2 | 0.4 | 3.7 |
| Stomach | 27.2 | 68.8 | 2.0 | 0.7 | 0.6 | 0.7 | 0.2 | BD | 4.3 |
| Aorta | 58.4 | 38.2 | 1.4 | BD | 1.7 | BD | 0.2 | BD | 3.3 |
| Brain | 9.8 | 83.1 | 4.5 | 1.0 | 2.5 | 1.1 | 0.5 | BD | 9.5 |
| Esophagus | 53.1 | 43.2 | 1.5 | BD | 1.4 | 0.6 | 0.2 | 0.2 | 3.9 |
| Spleen | 49.6 | 45.2 | 2.4 | BD | 1.0 | 1.1 | 0.3 | 0.8 | 5.6 |
| Testes | 57.1 | 40.4 | 1.1 | BD | 0.8 | 1.2 | BD | BD | 3.0 |
| Salivary gland | 11.1 | 85.5 | 1.9 | 3.6 | 0.2 | 0.2 | 0.1 | BD | 6.0 |
| Liver | 72.6 | 23.8 | 1.0 | BD | 1.7 | 0.8 | 0.2 | BD | 3.7 |
| Small intestine (duodenum) | 27.6 | 68.7 | 1.3 | BD | 0.6 | 1.8 | 0.1 | BD | 3.8 |
| Uterine horn | 45.3 | 52.4 | 0.8 | BD | 1.4 | 0.4 | 0.1 | BD | 2.6 |
| Kidney | 43.8 | 53.5 | 1.1 | 0.5 | 1.0 | 0.4 | 0.1 | 0.2 | 3.4 |
| Tongue | 51.5 | 45.8 | 1.2 | BD | 1.1 | 0.5 | n/d | BD | 2.8 |
| Heart | 43.2 | 53.3 | 1.8 | BD | 0.9 | 0.8 | 0.2 | 0.6 | 4.3 |
| Large intestine (colon) | 5.9 | 77.2 | 9.0 | 3.8 | BD | BD | 1.6 | 2.6 | 16.9 |
| Thymus | 52.1 | 44.8 | 1.1 | BD | 0.6 | 1.3 | 0.1 | BD | 3.1 |
| Pancreas | 38.5 | 59.1 | 1.2 | BD | 0.7 | 0.4 | 0.2 | BD | 2.5 |
a
Proportion of total Sia for each variant shown as a percentage, with the sum of all O-acetyl forms combined given in the far-right column (O-Ac sum). BD, the Sia form was below the limit of detection.