TABLE 3.
Analysis of A549 conditioned media and mucus collected from NHBE cell cultures for total sialic acid content using HPLC
| Cell type | % Siaa
|
||||||
|---|---|---|---|---|---|---|---|
| Source | Neu5Gc | Neu5Ac | Neu5,9Ac2 | Neu5,7Ac2 | Neu5,8Ac2 | Neu5,7,8/9Ac3 | |
| A549 | Conditioned mediab | BD | 98.16 | 1.84 | BD | BD | BD |
| NHBE | Mucus | BD | 98.6 | 1.40 | BD | BD | BD |
a
Proportion of total Sia for each variant shown as a percentage. BD, the Sia form was below the limit of detection. The percentages are averages of multiple conditioned-medium collections from A549 cells (n = 4) and multiple NHBE cell donors (n = 4).
b
Collected from A549 bronchial epithelial cell conditioned media; contained mucins.