FIG 2.

KIF1A protein concentration is reduced during PRV infection in neuronal cells. Differentiated PC12 cells were mock infected or infected with PRV Becker for 3, 8, 12, 16, 20, or 24 h at an MOI of 20. Cells were harvested at the time points shown, and lysates were analyzed by Western blotting to monitor protein levels of KIF1A, KIF5, KIF3A, and actin. Kinesin levels were measured by band intensities and normalized with respect to actin levels at each time point. Normalized values were again normalized to those of mock-infected 0-h samples. Viral protein expressions (PRV early gene EP0 and late genes MCP and US9) were also monitored by Western blotting at different times. Values are means plus SEMs (error bars) from four independent experiments. ***, P < 0.001; ****, P < 0.0001.