FIG 3.

The abundances of KIF1A, KIF5B, and GAPDH transcripts are significantly reduced in PRV-infected neuronal cells. Differentiated PC12 cells were infected with PRV Becker (A) or PRV Bartha (B) for 3, 8, 12, 16, 20, or 24 h at an MOI of 20. Cells were harvested at the time points shown. Total RNAs were extracted and qRT-PCR quantification was performed to measure KIF1A, KIF5B, and GAPDH mRNA levels using 18S rRNA as an internal control at each time point. The normalized values were then normalized to those of mock-infected 0-h samples. (C) The data from panels A and B are replotted to show the difference in the rate at which KIF1A and KIF5B transcript levels were reduced in PC12 cells infected with PRV Becker and PRV Bartha. Values are means plus SEMs (error bars) from three independent experiments. *, P < 0.05; **, P < 0.01.