TABLE 2.
Cryo-EM data collection and atomic model refinement statistics
| Parameter | PCV2-Fab complex | PCV2 |
|---|---|---|
| Data collection and reconstruction | ||
| EM equipment | FEI Talos F200C | FEI Talos F200C |
| Voltage (kV) | 200 | 200 |
| Detector | Ceta CCD (4,096 × 4,096) | Ceta CCD (4,096 × 4,096) |
| Pixel size (Å) | 1.14 | 1.14 |
| Electron dose (e−/Å2) | 35 | 35 |
| Defocus range (μm) | −1.5 to −3.0 | −1.0 to −3.0 |
| Reconstruction software | RELION 2.0 | RELION 3.0 |
| No. of collection micrographs | 2,774 | 433 |
| No. of particles used | 8,618 | 20,111 |
| Symmetry imposed | I | I |
| Final resolution (Å) | 7.2 | 6.7 |
| Model building software | Coot | Coot |
| Refinement software | Phenix | Phenix |
| Model refinement and validation | ||
| PDB accession no. | 6L62 | 6LM3 |
| EMDB accession no. | EMD-0838 | EMD-0916 |
| Root-mean-square deviation | ||
| Bonds (Å) | 0.01 | 0.01 |
| Angles (°) | 1 | 1 |
| Ramachandran plot (%) | ||
| Favored | 93.83 | 95.21 |
| Allowed | 6.17 | 4.79 |
| Outliers | 0 | 0 |
| Rotamer outliers | 0.55 | 0.58 |