TABLE 3.
Primers used for construction of the mutant clones
| Clone | Primer | Primer sequence |
|---|---|---|
| M-Y55A | Y55A-F | CCTGCACCTTCGGAGCGACTGTCAAGGCTAC |
| Y55A-R | GTAGCCTTGACAGTCGCTCCGAAGGTGCAGG | |
| M-T56A | T56A-F | GCACCTTCGGATATGCGGTCAAGGCTACCAC |
| T56A-R | GTGGTAGCCTTGACCGCATATCCGAAGGTGC | |
| M-K58A | K58A-F | CTTCGGATATACTGTCGCCGCTACCACAGTCAGA |
| K58A-R | TCTGACTGTGGTAGCGGCGACAGTATATCCGAAG | |
| M-A59S | A59S-F | GGATATACTGTCAAGTCCACCACAGTCAGAACG |
| A59S-R | CGTTCTGACTGTGGTGGACTTGACAGTATATCC | |
| M-T60A | T60A-F | GATATACTGTCAAGGCTGCGACAGTCAGAACGCC |
| T60A-R | GGCGTTCTGACTGTCGCAGCCTTGACAGTATATC | |
| M-T61A | T61A-F | CTGTCAAGGCTACCGCGGTCAGAACGCCCTC |
| T61A-R | GAGGGCGTTCTGACCGCGGTAGCCTTGACAG | |
| M-V62A | V62A-F | GTCAAGGCTACCACAGCCAGAACGCCCTCCTGG |
| V62A-R | CCAGGAGGGCGTTCTGGCTGTGGTAGCCTTGAC | |
| M-K88A | K88A-F | GGAGGGGGGACCAACGCCATCTCTATACCCTTTG |
| K88A-R | CAAAGGGTATAGAGATGGCGTTGGTCCCCCCTCC | |
| M-N128A | N128A-F | GCTGTTATTCTAGATGATGCGTTTGTAACTAAGGCCAC |
| N128A-R | GTGGCCTTAGTTACAAACGCATCATCTAGAATAACAGC | |
| M-T131A | T131A-F | CTAGATGATAACTTTGTAGCTAAGGCCACAGCCCTAACC |
| T131A-R | GGTTAGGGCTGTGGCCTTAGCTACAAAGTTATCATCTAG | |
| M-K132A | K132A-F | GATAACTTTGTAACTGCGGCCACAGCCCTAACC |
| K132A-R | GGTTAGGGCTGTGGCCGCAGTTACAAAGTTATC | |
| M-A133S | A133S-F | GATAACTTTGTAACTAAGTCCACAGCCCTAACCTATGAC |
| A133S-R | GTCATAGGTTAGGGCTGTGGACTTAGTTACAAAGTTATC | |
| M-T134A | T134A-F | CTTTGTAACTAAGGCCGCGGCCCTAACCTATGAC |
| T134A-R | GTCATAGGTTAGGGCCGCGGCCTTAGTTACAAAG | |
| M-A135S | A135S-F | GTAACTAAGGCCACATCCCTAACCTATGACCC |
| A135S-R | GGGTCATAGGTTAGGGATGTGGCCTTAGTTAC | |
| M-L136A | L136A-F | GTAACTAAGGCCACAGCCGCCACCTATGACCCCTATG |
| L136A-R | CATAGGGGTCATAGGTGGCGGCTGTGGCCTTAGTTAC | |
| M-T137A | T137A-F | CTAAGGCCACAGCCCTAGCCTATGACCCCTATGT |
| T137A-R | ACATAGGGGTCATAGGCTAGGGCTGTGGCCTTAG | |
| M-T189A | T189A-F | CTTTGGCTGAGACTACAAGCCTCGGCAAATGTGGAC |
| T189A-R | GTCCACATTTGCCGAGGCTTGTAGTCTCAGCCAAAG | |
| M-L231A | L231A-F | TAATCTTAAAGACCCCCCAGCCAAAACCCTAAATGAAT |
| L231A-R | ATTCATTTAGGGTTTTGGCTGGGGGGTCTTTAAGATTA | |
| PCV2a/LG-A59K | Forward | CGGATATACTGTCAAGAAAACCACAGTCAGAACGC |
| Reverse | GCGTTCTGACTGTGGTTTTCTTGACAGTATATCCG | |
| PCV2a/LG-K88P | Forward | AGGGGGGACCAACCCCATCTCTATACCCTTTG |
| Reverse | CAAAGGGTATAGAGATGGGGTTGGTCCCCCCT | |
| PCV2a/LG-T134N | Forward | TGTAACTAAGGCCAACGCCCTAACCTATG |
| Reverse | CATAGGTTAGGGCGTTGGCCTTAGTTACA | |
| PCV2b/2206-R59A | Forward | GATATACTATCAAGGCTACCACAGTCAGAAC |
| Reverse | GTTCTGACTGTGGTAGCCTTGATAGTATATC | |
| PCV2b/2206-P88K | Forward | GGGGCTCAAACAAACGCTCTGTGCC |
| Reverse | GGCACAGAGCGTTTGTTTTGAGCCCC | |
| PCV2d/2209-K59A | Forward | GTTATACTGTCAAGGCTACCACAGTCAGAAC |
| Reverse | GTTCTGACTGTGGTAGCCTTGACAGTATAAC | |
| PCV2d/2209-P88K | Forward | GGGGCTCAAACAAACTCACTGTGC |
| Reverse | GCACAGTGAGTTTGTTTGAGCCCC | |
| PCV2d/2209-N134T | Forward | CTTTGTAACAAAGGCCACAGCCCTAACCTATGAC |
| Reverse | GTCATAGGTTAGGGCTGTGGCCTTTGTTACAAAG |