FIG 5.

IRF5^−/− iPSCs, IRF5^Comp iPSCs, and Kolf2 iPSCs can be differentiated into iPS-DCs that display similar morphologies. IRF5^−/− iPSCs, IRF5^Comp iPSCs, and Kolf2 iPSCs were differentiated into dendritic cells using defined concentrations of growth factors to generate embryoid bodies (EBs) and GM-CSF and IL-4 to generate immature DCs from these EBs. (A) Total cell numbers of DC precursors harvested from DC differentiation plates. Data shown are from 8 independent differentiations per iPSC line. (B) Surface expression of DC markers was examined via flow cytometry in Kolf2, IRF5^−/−, and IRF5^Comp iPS-DCs. Representative plots are presented from one experiment, with experiments performed at least three times. (C) Gene expression of DC markers CD83 and CD86 and iPSC markers NANOG and POU5F1 by iPS-DCs relative to GAPDH was quantified using TaqMan gene expression assays. The data shown represent four technical replicates per assay, with assays repeated at least twice from independent iPS-DC batches. (D) Morphologies of iPS-DCs generated from Kolf2, IRF5^Comp, and IRF5^−/− iPSCs.