FIG 7.

Type I IFN signaling enhances IL-6 and TNF-α production by iPS-DCs. A total of 2 × 10⁴ iPS-DCs were challenged as stated below for each assay, and supernatants were harvested after 24 h, unless otherwise stated. A/X-31 influenza (IAV) was used at an MOI of 1. (A) Cells were preincubated for 1 h with anti-IFNAR1 antibody or left untreated prior to viral infection. Data shown represent the mean ±SEM for triplicate wells from at least 3 experiments. Supernatants were harvested and assayed for IL-6 and TNF-α by ELISA. (B) Supernatants from mock or IAV-infected Kolf2 or IRF5^−/− iPS-DCs were harvested at 24 h and assayed for IFN-α and IFN-β by ELISA. Data shown represent 2 separate experiments.