TABLE 1.
Cryo-EM data collection, refinement, and validation statistics
| Parameter or statistic | CtenRNAV-II VPs (EMD-10200 and PDB 6SHL) |
|---|---|
| Data collection and processing | |
| Magnification | 140,000 |
| Voltage (kV) | 300 |
| Electron exposure (e–/Å2) | 29 |
| Defocus range (μm) | –1.00 to –3.00 |
| Pixel size (Å) | 1.06 |
| Symmetry imposed | I |
| No. of initial particle images | 9,920 |
| No. of final particle images | 8,315 |
| Map resolution (Å) | 3.1 |
| FSC threshold | 0.143 |
| Map resolution range (Å) | 2.3–4.1 |
| Refinement | |
| Map sharpening B factor (Å2) | –129.95 |
| Model composition | |
| Nonhydrogen atoms | 378,240 |
| Protein residues | 48,600 |
| B factors (Å2) | |
| Protein (min/max/mean) | 12.53/51.60/21.05 |
| RMSDa | |
| Bond lengths (Å) | 0.009 |
| Bond angles (°) | 1.386 |
| Validation | |
| MolProbity score | 1.78 |
| Clashscore | 4.54 |
| Poor rotamers (%) | 0.88 |
| Ramachandran plot | |
| Favored (%) | 89.90 |
| Allowed (%) | 9.85 |
| Disallowed (%) | 0.25 |
| CC (main chain/side chain) | 0.87/0.85 |
| EMRinger score | 4.51 |
| d 99 (masked/unmasked) | 3.2/3.1 |
a
RMSD, root mean square deviation.