REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial Strains
Escherichia coli K12 AB1157	Bachmann, 1996	CGSC#1157
AB1157 (Ab246) mukB(D1406A)-mYpet-kan	Badrinarayanan et al., 2012a	N/A
AB1157 (SN191) lacO240-hyg at L3 (2268 kb) tetO240-gen at R3 (852 kb) ΔleuB::Plac-lacI-mCherry-frt ΔgalK::Plac-tetR-mCerulean-frt mukB-mYpet-frt	Lab collection	N/A
AB1157 (SN192) lacO240-hyg at ori1 (3908 kb) tetO240-gen at ter3 (1644 kb) ΔleuB::Plac-lacI-mCherry-frt ΔgalK::Plac-tetR-mCerulean-frt mukB-mYpet-frt	Nolivos et al., 2016	N/A
AB1157 (SN301) lacO240-hyg at L3 (2268 kb) tetO240-gen at R3 (852 kb) ΔleuB::Plac-lacI-mCherry-frt ΔgalK::Plac-tetR-mCerulean-frt mukB-mYpet-frt ΔmatP::cat	Lab collection	N/A
AB1157 (SN302) lacO240-hyg at ori1 (3908 kb) tetO240-gen at ter3 (1644 kb) ΔleuB::Plac-lacI-mCherry-frt ΔgalK::Plac-tetR-mCerulean-frt mukB-mYpet-frt ΔmatP::cat	Nolivos et al., 2016	N/A
AB1157 (SN311) lacO240-hyg at ori1 (3908 kb) tetO240-gen at ter3 (1644 kb) ΔleuB::Plac-lacI-mCherry-frt ΔgalK::Plac-tetR-mCerulean-frt mukB(E1407Q)-mYpet-kan	Nolivos et al., 2016	N/A
AB1157 (JM41) mukB-HaloTag-kan	Banaz et al., 2019	N/A
AB1157 (JM56) mukB-HaloTag-frt ΔmukE::kan	This study	N/A
AB1157 (JM90) lacO240-hyg at ori1 (3908 kb) tetO240-gen at ter3 (1644 kb) ΔleuB::Plac-lacI-mCherry-frt ΔgalK::Plac-tetR-mCerulean-frt kan-araC-Para-smtA-mukFE-mukB-mYpet-frt	This study	N/A
AB1157 (JM91) lacO240-hyg at ori1 (3908 kb) tetO240-gen at ter3 (1644 kb) ΔleuB::Plac-lacI-mCherry-frt ΔgalK::Plac-tetR-mCerulean-frt kan-araC-Para-smtA-mukFE-mukB-mYpet-frt, ΔmatP::cat	This study	N/A
AB1157 (JM97) lacO240-hyg at ori1 (3908 kb) tetO240-gen at ter3 (1644 kb) ΔleuB::Plac-lacI-mCherry-frt ΔgalK::Plac-tetR-mCerulean-frt frt-araC-Para-smtA-mukFE-mukB(E1407Q)-mYpet-kan	This study	N/A
AB1157 (JM101) lacO240-hyg at L3 (2268 kb) tetO240-gen at R3 (852 kb) ΔleuB::Plac-lacI-mCherry-frt ΔgalK::Plac-tetR-mCerulean-frt kan-araC-Para-smtA-mukFE-mukB-mYpet-frt	This study	N/A
AB1157 (JM103) frt-araC-Para-smtA-mukFE-mukB-HaloTag-kan	This study	N/A
AB1157 (JM124) lacO240-hyg at ori1 (3908 kb) tetO240-gen at ter3 (1644 kb) ΔleuB::Plac-lacI-mCherry-frt ΔgalK::Plac-tetR-mCerulean-frt frt-araC-Para-smtA-mukFE-mukB(D1406A)-mYpet-kan	This study	N/A
Chemicals, Peptides, and Recombinant Proteins
DL-serine hydroxamate	Sigma-Aldrich	Cat#S4503
Deposited Data
Raw and analyzed data	This study	https://doi.org/10.17632/d7p7hk3zzv.1
Oligonucleotides
PCR primers	Eurogentec	See Table S1
Software and Algorithms
MATLAB	MathWorks	https://uk.mathworks.com/products/matlab.html
SuperSegger	Stylianidou et al., 2016	https://github.com/wiggins-lab/SuperSegger/wiki
NIS-Elements AR	Nikon	https://www.microscope.healthcare.nikon.com
SoftWoRx	GE Healthcare	https://www.gelifesciences.com/en/gb/shop/cell-imaging-and-analysis/high-and-super-resolution-microscopes/instruments/deltavision-omx-sr-imaging-system-p-03020
Chromagnon	Matsuda et al., 2018	https://github.com/macronucleus/chromagnon
ImageJ	Schneider et al., 2012	https://imagej.nih.gov/ij/
SIMcheck	Ball et al., 2015	https://github.com/MicronOxford/SIMcheck
3Dscript	Schmid et al., 2019	https://imagej.net/3Dscript
SGNS2	Lloyd-Price et al., 2012	https://sites.google.com/view/andreribeirolab/home/software