Fig. 3.

Clinical-grade WJ-MSC characterisation. (a) Representative microscopic image of expanded WJ-MSCs culture. Scale bar = 100 µm. (b) Normal karyotype of a DP, corresponding to a representative cell line. (c) Immunophenotypic characteristics of four clinical-grade cell batches and average expression (mean ± SEM). (d) Immunomodulation potential measured in four batches of clinical-grade WJ-MSCs, as the capacity to inhibit proliferation of polyclonal stimulated lymphocytes. (e) Microscopic images showing stimulated PBMC clumping, and clump reduction when co-cultured with WJ-MSCs. Arrowheads indicate clumps. Scale bars = 100 µm. PBMCs, peripheral blood mononuclear cells; WJ-MSCs, Wharton's jelly mesenchymal stromal cells.