Table 4.

Mutational Spectra of +1 bp and -1 bp Systems by DNA Sequencing

Microsatellites	Mutational spectra (% of colonies)
	-1 bp OF system	+1 bp OF system
D9S242 (AAAG)18	36.0% deletions	71.7% deletions
	24.2% one-repeat	93.2% one-repeat
	48.5% two-repeat	6.7% two-repeat
	13.6% three-repeat	9.0% heteroduplexes
	13.6% five or more repeat	15.7% wild type
	15.3% heteroduplexes	3.6% one-repeat insertions
	25.1% wild type
	23.5% insertions
	93.0% one-repeat
	7.0% four or more repeat insertions
D9S242 (AAAG)15	37.5% deletions	70.2% deletions
	28.6% one-repeat	95.7% one-repeat
	71.4% two-repeat	4.3% two-repeat
	26.8% heteroduplexes	11.9% heteroduplexes
	32.1% wild type	17.9% wild type
	3.6% one-repeat insertions
D9S242 (AAAG)12	4.1% deletions	76.7% deletions
	40.0% one-repeat	94.6% one-repeat
	60.0% two-repeat	5.4% two-repeat
	38.8% heteroduplexes	4.1% heteroduplexes
	40.8% wild type	19.2% wild type
	16.3% one-repeat insertions

NOTE. Wild-type refers to the native sequence in the construct. Each repeat unit is 1 AAAG.

OF, out-of-frame.