Table 2.
Antibody sources and dilutions.
| Antibodies | Source | Catalog | Dilution ratio |
|---|---|---|---|
| Primary antibodies for Western blotting | |||
| Anti-sEH monoclonal antibody | Abcam | ab155280 | 1: 1000 |
| Anti-COX-2 polyclonal antibody | Proteintech | 12375-1-AP | 1: 2000 |
| Anti-Nrf2 monoclonal antibody | CST | #12721 | 1: 1000 |
| Anti-TREM-1 polyclonal antibody | Proteintech | 11791-1-Ap | 1: 1000 |
| Anti-NLRP3 monoclonal antibody | CST | #15101 | 1: 2000 |
| Anti-IL-1β polyclonal antibody | R&D | AF-401-NA | 1: 2000 |
| Anti-pro-caspase-1/p10/p20 monoclonal antibody | Abcam | Ab179515 | 1: 1000 |
| Anti-I-κBα monoclonal antibody | Abcam | Ab32518 | 1:2500 |
| Secondary antibodies for Western blotting | |||
| Anti-β-actin polyclonal antibody | SAB | #21338 | 1: 7500 |
| Anti-β-tubulin monoclonal antibody | Servicebio | #48885 | 1: 2000 |
| Antibodies for IP assay | |||
| Anti-NLRP3 monoclonal antibody | AdipoGen | AG-20B-0014-C100 | 1:1000 |
| Anti-ASC monoclonal antibody | CST | 67824S | 1:1000 |