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. 2020 Mar 26;10(11):4822–4838. doi: 10.7150/thno.42387

Figure 7.

Figure 7

RSV improves SIRT1-relied mitochondrial biogenesis process in NaF-injured hippocampal tissues of offspring rats. (A) Immunoblot analyses of SIRT1, PGC-1 and NRF1 in hippocampal tissues (n = 3 rats per group). GAPDH was used as the internal control. (B) Representative images of the IHC staining for SIRT1+ and NRF1+ neurons in hippocampal DG region. SIRT1+ and NRF1+ neuronal cells are demonstrated by black arrows and quantified. Scale bars represent 50 μm, n = 2 rats per group. (C) RT-qPCR analyses of relative mtDNA contents in hippocampal tissues (n = 6 rats per group). (D) Immunoblot analyses of ATP6 in hippocampal tissues (n = 3 rats per group). (E) Representative images of the IHC staining for ATP6+ neurons in hippocampal DG region. ATP6+ neuronal cells are demonstrated by black arrows and quantified. Scale bars represent 50 μm, n = 2 rats per group. Data information: Data are presented as mean ± SD. * P < 0.05 is considered significant compared with Control, # P < 0.05 is significantly different from NaF group and &P < 0.05 is considered significant from NaF+RSV group by one-way ANOVA test.