Figure 2.

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 Purification of the mouse liver 40‐kDa protein recognized by serum from MHV‐infected mice. The arrows indicate the fractions containing the protein. (A) DEAE‐cellulose chromatography of mouse liver 100,000×g supernatant. Elution was done in buffer Tris‐HCl 20 mM, pH 8.0, and a 0–0.3 M NaCl gradient (dotted line). (B) The material isolated from the fraction indicated in (A) was chromatographied in a Sephadex G‐100 column equilibrated with Tris 5 mM, NaCl 150 mM, pH 7.4.