Figure 3.

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 Western blot analysis (A) and Coomasie blue staining (B) of different mouse liver fractions. According with Sect. 4 and Fig. 2: liver supernatant obtained after 100,000×g centrifugation (lane 1), DEAE‐cellulose (lane 2) or Sephadex G‐100 chromatography (lane 3). SDS‐PAGE was performed in 14×16 cm gels using a gradient of 10‐12.5% of polyacrylamide. Transferred proteins were incubated with serum from non‐infected (‐) or MHV‐infected (+) mice. The positions of molecular mass markers (kDa) are shown at right.