Table 2.
Structure | Target | B2 | Bspace | B1 | F1 | Fspace | F2 |
---|---|---|---|---|---|---|---|
Stem and Loop ×1 | 1 | 1 | 1 | 1 | 2 | 1 | 1 |
Stem and Loop ×2 | 2 | 2 | 2 | 3 | 2 | 1 | 1 |
Stem and Loop ×3 | 3 | 2 | 2 | 3 | 4 | 2 | 2 |
Stem and Loop ×4 | 4 | 3 | 3 | 5 | 4 | 2 | 2 |
Stem and Loop ×5 | 5 | 3 | 3 | 5 | 6 | 3 | 3 |
Stem and Loop ×6 | 6 | 4 | 4 | 7 | 6 | 3 | 3 |
Stem and Loop ×7 | 7 | 4 | 4 | 7 | 8 | 4 | 4 |
Bspace: sequence between B1 and B2 sequences in backward inner primer
Fspace: sequence between F1 and F2 sequences in forward inner primer
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