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Fluorescent immunohistochemical detection of c‐Fos and GFP‐NPY neurons coexpressed in the hypothalamic arcuate nucleus at 60 min after saline (A‐C) or risperidone (D‐F) treatment. A and D, Green staining shows NPY neurons expressing GFP; (B, E): red staining shows c‐Fos‐positive neurons. C and F, Yellow staining indicating that the neurons express both c‐fos and NPY; C'&F' is higher magnification of the boxed area in C&F. Scale bars in A‐F is 80 μm and in C'&F' is 25 μm
