Skip to main content
. Author manuscript; available in PMC: 2020 Apr 17.
Published in final edited form as: Biochemistry. 2019 Mar 20;58(13):1705–1708. doi: 10.1021/acs.biochem.9b00131

Figure 3.

Figure 3.

Fluorescence correlation spectroscopy (FCS) autocorrelation curves of eGFP-tagged membrane proteins. FCS recordings were made on the plasma membrane of transiently transfected HEK293 cells expressing the indicated eGFP-tagged proteins. These graphs are representative examples taken from one of three independent studies with multiple samples. (A) Fluorescence intensity fluctuations from which recordings outside the range of 50–250 kHz were excluded. (B) Autocorrelation curve of FCS data (red line) with a model curve fitting a two-dimensional two-component model (blue line). Diffusion times for the fast (τD1) and slow (τD2) components are shown.